DB772hydrate
PRODUCTS SPECIFICATIONS
DESCRIPTION
Overview
| MDL | - |
|---|---|
| Molecular Weight | 502.54 |
| Molecular Formula | C20H19ClN4O2 |
| SMILES | [H]Cl.C1(C2=CC=C(C3=CC=C(C4=NCCN4)C=C3)O2)=NC5=CC=CC=C5N1.[2.8].[4].O |
Summary
DB772 hydrate is a bovine viral diarrhoea virus ( BVDV ) inhibitor. DB772 also has anti-prion activity [1] .
In Vitro
DB772 hydrate (0-100 μM, 96 hours) can inhibit replication of the viruses BVDV2 and BDV at concentrations greater than 0.2 μM, Bungowannah virus replication at concentrations greater than 0.01 μM, and HoBi virus replication greater than 1.65 μM
[2]
.
DB772 hydrate has antiprion activity with CC
50
(50% cytotoxic concentration) value of 3.8 μM, TC-EC
50
(50% tissue culture effective concentration) value of 0. 8 μM and inhibits PrP
Sc
accumulation in sheep microglia
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [3]
| Cell Line: | PrP Sc -positive and -negative cell lines of human telomerase-immortalized (hTERT) ovine microglia |
| Concentration: | 1 μM |
| Incubation Time: | 4 days |
| Result: |
Showed no significant effect on cell viability with 96.4% cell viability.
Significantly reduced PrP Sc -positive spots by 65.8% and completely inhibited PrP Sc accumulation in scrapie-infected primary ovine microglia at 4 μM. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : ≥ 4 mg/mL ( 7.96 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.9899 mL | 9.9495 mL | 19.8989 mL |
| 5 mM | 0.3980 mL | 1.9899 mL | 3.9798 mL |
| 10 mM | --- | --- | --- |
References
- [1] James B Stanton, et al. Discovery of a novel, monocationic, small-molecule inhibitor of scrapie prion accumulation in cultured sheep microglia and Rov cells. PLoS One. 2012;7(11):e51173.
- [2] Benjamin W Newcomer, et al. Efficacy of an antiviral compound to inhibit replication of multiple pestivirus species. Antiviral Res. 2012 Nov;96(2):127-9.
- [3] Kelcey D Dinkel, et al. Antiprion Activity of DB772 and Related Monothiophene- and Furan-Based Analogs in a Persistently Infected Ovine Microglia Culture System. Antimicrob Agents Chemother. 2016 Aug 22;60(9):5467-82.