[CAS NO. 3033087-05-4] hDHODH-IN-10
PRODUCTS SPECIFICATIONS [3033087-05-4]
DESCRIPTION [3033087-05-4]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 470.80 |
| Molecular Formula | C21H15ClF4N2O4 |
| SMILES | C/C(O)=C(C(NC1=C(C=C(C(O[C@H](C(F)(F)F)C)=C1)C(C2=C(C=CC=C2)Cl)=O)F)=O)\C#N |
Summary
hDHODH-IN-10 is a selective, potent and orally active hDHODH inhibitor, with an IC 50 value of 10.9 nM. hDHODH-IN-10 forms hydrogen bonds with key residues Arg136 and Gln47. hDHODH-IN-10 inhibits the proliferation of cancer cells. hDHODH-IN-10 can be used in the research of cancers, such as AML, colorectal cancer [1] .
IC50 & Target
IC50: hDHODH (10.9 nM) [1] .
In Vitro
hDHODH-IN-10 (compound 7d, 1 nM-100 μM) displays anti-proliferative activities against multiple human cancer cells
[1]
.
hDHODH-IN-10 (0.0625-0.25 μM, 24 h) increases the percentage of S-phase cells in Raji and HCT116 cells
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | U937, HCT116, A375, Kasumi-1 and KG-1 cells |
| Concentration: | 0-10 μM approximately |
| Incubation Time: | 96 h |
| Result: | Inhibits cell proliferation with IC 50 values of 0.1-0.8 μM. |
Cell Cycle Analysis [1]
| Cell Line: | Raji and HCT116 cells |
| Concentration: | 0.0625, 0.125 and 0.25 μM |
| Incubation Time: | 24 h |
| Result: | Increased the percentage of S-phase cells from 42.8% to 54.2%, 60.6% and 67.1%, respectively. |
In Vivo
hDHODH-IN-10 (compound 7d, 30 mg/kg, oral administration) inhibits tumor growth in Raji and HCT116 cells xenograft mice model
[1]
.
hDHODH-IN-10 (500 mg/kg, oral administration) exhibits a favorable safety profile
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: | Raji and HCT116 cells xenograft mice model [1] |
| Dosage: | 30 mg/kg |
| Administration: | Oral administration |
| Result: | Showed a tumor growth inhibitory (TGI) rate of 58.3% (Raji model) and 42.1% (HCT116 model). |
| Animal Model: | BALB/c mice (acute toxicity assay) [1] |
| Dosage: | 500 mg/kg |
| Administration: | Oral administration |
| Result: |
LD
50
is about 500 mg/kg.
Induced a weak dysfunction of liver. |
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.