ReACp53
PRODUCTS SPECIFICATIONS
DESCRIPTION
Overview
| MDL | - |
|---|---|
| Molecular Weight | 2617.13 |
| Molecular Formula | C108H206N52O24 |
| SMILES | - |
Summary
ReACp53 could inhibit p53 amyloid formation and rescue p53 function in cancer cell lines.
IC50 & Target
p53 amyloid formation [1] .
In Vitro
ReACp53 penetrates into HGSOC primary cancer cells and converts mutant p53 from a punctate state into soluble WT-like p53. ReACp53 also induces cancer cell death, cell cycle arrest and results in p53 degradation. ReACp53 specifically affects cell viability and proliferation of cancer cells bearing mutant p53 but not wild type when grown as organoids [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Only mutant p53-bearing tumors in the ReACp53-treated mice cohorts are 80-90% smaller in weight than the control cohort, confirming the ability of ReACp53 to limit tumor proliferation and shrink tumors. A significant reduction of Ki67 positive cells is evident in ReACp53-treated OVCAR3 xenografts, indicative of a reduced proliferative index. Similar results are observed in the minimal residual disease model. In the paradigm, administration of ReACp53 results in a significant increase in p21 and MDM2 transcription in OVCAR3 but not MCF7 xenografts. A significantly increased population is also found in G0/G1 phase, supporting proliferative arrest upon ReACp53 administration in vivo [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Sealed storage, away from moisture
| Powder | -80°C | 2 years |
|---|---|---|
| -20°C | 1 year |
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL ( 38.21 mM ; Need ultrasonic)
H 2 O : 25 mg/mL ( 9.55 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 0.3821 mL | 1.9105 mL | 3.8210 mL |
| 5 mM | 0.0764 mL | 0.3821 mL | 0.7642 mL |
| 10 mM | 0.0382 mL | 0.1910 mL | 0.3821 mL |
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1.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (19.10 mM); Clear solution; Need ultrasonic
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2.
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3.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (0.96 mM); Clear solution
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4.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: ≥ 2.5 mg/mL (0.96 mM); Clear solution