[CAS NO. ] Orexin B, human TFA

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PRODUCTS SPECIFICATIONS

Catalog

HY-P1339A

Brand

MCE

CAS

-

DESCRIPTION

Overview

MDL	-
Molecular Weight	3013.36
Molecular Formula	C123H212N44O35S.C2HF3O2
SMILES	-

For research use only. We do not sell to patients.

Summary

Orexin B, human (TFA) is an endogenous agonist at Orexin receptor with K _i s of 420 and 36 nM for OX1 and OX2, respectively.

IC50 & Target

Ki: 420 nM (OX1), 36 nM (OX2) ^[1] .

In Vitro

Orexin B is derived by proteolytic amino acid precursor, prepro-orexin, which is encoded by a gene localized to chromosome 17q21 in humans. In radioligand binding studies, Orexin B has a higher affinity for the OX2 receptor ^[1] .
Human Orexin B has two amino acid substitutions when compared with the rodent sequence. OX2 receptor is indeed a high-affinity receptor for human orexin B, with an IC ₅₀ of 36 nM in the binding assay and an EC ₅₀ of 60 nM in the [Ca ²⁺ ]i transient assay. Human Orexin B has significantly lower affinity for the human OX1: the calculated IC ₅₀ in the competitive binding assay and the EC ₅₀ in the [Ca ²⁺ ]i transient assay are 420 nM and 2500 nM for human orexin-B, respectively ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Human Orexin B significantly augments food intake; at the 2 hr time point, 5- and 12-fold stimulation of food consumption is observed by 3 nM and 30 nM orexin-B, respectively ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar rats (180-200 g) ^[2] .
Dosage:	3 nM and 30 nM.
Administration:	Administered in a 5 mL bolus through a catheter placed in the left lateral ventricle in early light phase.
Result:	Significantly augmented food intake.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H ₂ O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH ₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH ₄ OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.