Orexin B, rat, mouse TFA
PRODUCTS SPECIFICATIONS
DESCRIPTION
Overview
| MDL | - |
|---|---|
| Molecular Weight | 3050.42 |
| Molecular Formula | C₁₂₈H₂₁₆F₃N₄₅O₃₆S |
| SMILES | - |
Summary
Orexin B, rat, mouse (Rat orexin B) TFA is an endogenous orexin receptor agonist. Orexin B, rat, mouse TFA binds and activates two closely related orphan G protein-coupled receptors OX1-R and OX2-R. Orexin B, rat, mouse TFA stimulates food intake and energy expenditure and plays a significant role in sleep-wakefulness regulation [1] [2] [3] .
In Vitro
OX2 receptor is indeed a high-affinity receptor for human orexin B, with an IC 50 of 36 nM in the binding assay and an EC 50 of 60 nM in the [Ca 2+ ]i transient assay. Human Orexin B has significantly lower affinity for the human OX1: the calculated IC 50 in the competitive binding assay and the EC 50 in the [Ca 2+ ]i transient assay are 420 nM and 2500 nM for human orexin-B, respectively [2] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Orexin-B modulates the activity of rod bipolar cells (RBCs) located in the outer retina of rat. Intravitreal injection of orexin-B increased the amplitude of the scotopic electroretinographic b-wave, a reflection of RBC activity, recorded in vivo [4] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Sealed storage, away from moisture
| Powder | -80°C | 2 years |
|---|---|---|
| -20°C | 1 year |
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H 2 O : 100 mg/mL ( 32.78 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 0.3278 mL | 1.6391 mL | 3.2782 mL |
| 5 mM | 0.0656 mL | 0.3278 mL | 0.6556 mL |
| 10 mM | 0.0328 mL | 0.1639 mL | 0.3278 mL |
References
- [1] Smart D, et al. Orexins: a new family of neuropeptides. Br J Anaesth. 1999 Nov;83(5):695-7.
- [2] Sakurai T, et al. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell. 1998 Feb 20;92(4):573-85.
- [3] Ziolkowska A, et al. Orexin B inhibits proliferation and stimulates specialized function of cultured rat calvarial osteoblast-like cells. Int J Mol Med. 2008;22(6):749-755.
- [4] Zhang G, et al. Orexin-B modulates synaptic transmission of rod bipolar cells in rat retina. Neuropharmacology. 2018;133:38-50.