[CAS NO. ] CRF, bovine TFA

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PRODUCTS SPECIFICATIONS

Catalog

HY-P1533A

Brand

MCE

CAS

-

DESCRIPTION

Overview

MDL	-
Molecular Weight	4811.36
Molecular Formula	C208H341F3N60O65S
SMILES	-

For research use only. We do not sell to patients.

Summary

CRF, bovine (TFA) is a potent agonist of CRF receptor , and displaces [ ¹²⁵ I-Tyr]ovine CRF with a K _i of 3.52 nM ^[1] .

IC50 & Target

Ki: 3.52 nM (CRF receptor) ^[1] .

In Vitro

CRF, bovine is a potent agonist of CRF receptor, and displaces [ ¹²⁵ I-Tyr]ovine CRF with a K _i of 3.52 nM ^[1] . CRF shows pEC ₅₀ s of 11.16, 8.53 and 8.70 for human CRF ₁ , human CRF ₂ and rat CRF _2α ^[2] . CRF is released from hypothalamic-pituitary-adrenal (HPA) axis induced by stress, and leads to production of glucocorticoids which down regulate immune responses. CRF also has proinflammatory effects. CRF affects brain microvessel endothelial cells (BMEC) structure or function, CRF (100 nM) significantly increases cAMP in BMEC ^[3] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H ₂ O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH ₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH ₄ OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.