[CAS NO. 1093793-05-5] PV-1019
PRODUCTS SPECIFICATIONS [1093793-05-5]
DESCRIPTION [1093793-05-5]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 394.39 |
| Molecular Formula | C18H18N8O3 |
| SMILES | O=C(C(N1)=CC2=C1C([N+]([O-])=O)=CC=C2)NC3=CC=C(/C(C)=N/NNC(N)=N)C=C3 |
Summary
IC50 & Target
|
Chk2 24 nM (IC 50 ) |
In Vitro
PV-1019 (NSC 744039; 0.1-100 μM; 48 h) has antiproliferative effect in human tumor cells
[1]
.
PV-1019 inhibits Chk2 autophosphorylation and histone H1 phosphorylation with an IC
50
value of 138 nM
[1]
.
PV-1019 (1-50 μM; 1 h; OVCAR-5 cells) inhibits the
Topotecan
-induced Chk2 autophosphorylation with an IC
50
value of 2.8 μM
[1]
.
PV-1019 (1 μM; 16 h) abrogates IR-induced apoptosis in mouse thymocytes
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | human tumor cells |
| Concentration: | 0.1-100 μM |
| Incubation Time: | 48 hours |
| Result: | Inhibited cell growth in a dose-dependent manner. |
Western Blot Analysis [1]
| Cell Line: | OVCAR-5 cells |
| Concentration: | 1, 5, 10, and 25 μM |
| Incubation Time: | 1 hours |
| Result: | Decreased the level of Chk2 autophosphorylation (Ser516) in a dose-dependent manner. |
Cell Cycle Analysis [1]
| Cell Line: | mouse thymocytes |
| Concentration: | 1 μM |
| Incubation Time: | 16 hours |
| Result: | Decreased in the sub-G1 fraction (56%). |
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.