[CAS NO. 2924274-19-9] TJ08
PRODUCTS SPECIFICATIONS [2924274-19-9]
DESCRIPTION [2924274-19-9]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 405.38 |
| Molecular Formula | C22H16FN3O4 |
| SMILES | COC(C1=CC=C2C(N=C(N2CC3=CC=CC=C3)C4=CC=C(C([N+]([O-])=O)=C4)F)=C1)=O |
Summary
In Vitro
TJ08 (0-20 μM;48 and 72 h) has antiproliferative effect on various cancer cell lines with IC
50
s ranging from 1.88 to 3.82 μM
[1]
.
TJ08 (1-10 μM; 24 h) instigates apoptosis by permuting mitochondrial membrane potential
[1]
.
TJ08 (1-10 μM; 24 h) instigates S phase arrest and abrogates cancer cell progression
[1]
.
TJ08 (1-10 μM; 24 h) causes the upregulation of cleaved caspase and downregulation of antiapoptotic BCl
2
proteins
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Proliferation Assay [1]
| Cell Line: | Human leukemic cancer cells (Jurkat, K562, and Molt4), human cervical cancer cells (HeLa), human colorectal carcinoma cells (HCT116), and human pancreatic ductal adenocarcinoma (MIAPaCa-2) |
| Concentration: | 0-20 μM |
| Incubation Time: | 48 and 72 h |
| Result: | Showed effective against various cells with IC 50 s of 1.88 μM, 1.89 μM, 2.05 μM, 2.11 μM, 3.04 μM, and 3.82 μM against Jurkat, K562, MOLT-4, HeLa, HCT116, and MIA PaCa-2 cancer cell lines, respectively. |
Apoptosis Analysis [1]
| Cell Line: | Jurkat cells |
| Concentration: | 1, 5, 10 μM |
| Incubation Time: | 24 h |
| Result: | Induced cell death by apoptosis, not by necrosis, in a concentration-dependent manner. |
Cell Cycle Analysis [1]
| Cell Line: | Jurkat cells |
| Concentration: | 1, 5, 10 μM |
| Incubation Time: | 24 h |
| Result: | Cauesd the accumulation of cells at the S phase in a concentration-dependent manner followed by increased accumulation of a sub-G1 population of cells. |
Western Blot Analysis [1]
| Cell Line: | Jurkat cells |
| Concentration: | 1, 5, 10 μM |
| Incubation Time: | 24 h |
| Result: | Caused the upregulation of cleaved caspase and downregulation of antiapoptotic BCl 2 proteins. |
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
