TAT TFA
PRODUCTS SPECIFICATIONS
DESCRIPTION
Overview
| MDL | - |
|---|---|
| Molecular Weight | 1673.85 |
| Molecular Formula | C66H119N32F3O16 |
| SMILES | - |
Summary
TAT TFA (YGRKKRRQRRR) is derived from the transactivator of transcription (TAT) of human immunodeficiency virus (HIV-1) and is a cell-penetrating peptide. TAT can increase the yields and the solubility of heterologous proteins [1] .
IC50 & Target
|
HIV-1 |
In Vitro
TAT (YGRKKRRQRRR) represents amino acids 47-57 of the human immunodeficiency virus type I (HIV-1) TAT protein. The positively charged protein transduction domain of the HIV-1 TAT protein (TAT-PTD; residues 47-57 of TAT) rapidly translocates across the plasma membrane of living cells. TAT (YGRKKRRQRRR; TAT-PTD) can cross the hydrophobic lipid bilayer of a cell membrane simply by passive diffusion [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Sealed storage, away from moisture
| Powder | -80°C | 2 years |
|---|---|---|
| -20°C | 1 year |
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H 2 O : 50 mg/mL ( 29.87 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 0.5974 mL | 2.9871 mL | 5.9743 mL |
| 5 mM | 0.1195 mL | 0.5974 mL | 1.1949 mL |
| 10 mM | 0.0597 mL | 0.2987 mL | 0.5974 mL |
-
1.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (59.74 mM); Clear solution; Need ultrasonic