Fz7-21 TFA
PRODUCTS SPECIFICATIONS
DESCRIPTION
Overview
| MDL | - |
|---|---|
| Molecular Weight | 1910.07 |
| Molecular Formula | C85H115N18F3O25S2 |
| SMILES | - |
1 Publications Citing Use of MCE
Summary
Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA is a potent peptide antagonist of FZD7 receptors , selectively binds to FZD7 CRD subclass and alters the conformation of the CRD and the architecture of its lipid-binding groove. The EC 50 values are 58 and 34 nM for human and mouse FZD7 CRD, respectively. Fz7-21 TFA impairs the function of FZD7 in Wnt-β-catenin signalling and stem cell function in intestinal organoids [1] [2] .
IC50 & Target
EC50: 58 nM (human FZD7 CRD), 34 nM (mouse FZD7 CRD) [1]
In Vitro
Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA (0-100 μM; 6 h; HEK293-TB cells) impairs Wnt signaling with IC
50
value of 100 nM
[1]
.
Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA (1 µM) blocks WNT3A-mediated stabilization of β-catenin in mouse L cells with IC
50
value of 50 nM
[1]
.
Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA (200 µM; 48 h; LGR5–GFP
+
stem cells) disrupts LGR
5+
stem cell function
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Sealed storage, away from moisture
| Powder | -80°C | 2 years |
|---|---|---|
| -20°C | 1 year |
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 33.33 mg/mL ( 17.45 mM ; Need ultrasonic)
H 2 O : < 0.1 mg/mL (insoluble)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 0.5235 mL | 2.6177 mL | 5.2354 mL |
| 5 mM | 0.1047 mL | 0.5235 mL | 1.0471 mL |
| 10 mM | 0.0524 mL | 0.2618 mL | 0.5235 mL |
References