Dabcyl-KTSAVLQSGFRKME-Edans TFA
PRODUCTS SPECIFICATIONS
DESCRIPTION
Overview
| MDL | - |
|---|---|
| Molecular Weight | 2195.44 |
| Molecular Formula | C97H142F3N25O26S2 |
| SMILES | - |
1 Publications Citing Use of MCE
Summary
In Vitro
Recombinant 3CL
pro
is expressed in Pichia pastoris GS115 as a 42 kDa protein that displays a K
m
of 15 μM with Dabcyl-KTSAVLQSGFRKME-Edans TFA as substrate
[1]
.
The Dabcyl-KTSAVLQSGFRKME-Edans TFA fluorogenic peptide is used as the substrate and the enhanced fluorescence due to cleavage of this substrate catalyzed by the protease is monitored at 538 nm with an excitation wavelength of 355 nm using a fluorescence plate reader
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Sealed storage, away from moisture and light, under nitrogen
| Powder | -80°C | 2 years |
|---|---|---|
| -20°C | 1 year |
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Solvent & Solubility
H 2 O : 6.67 mg/mL ( 3.04 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 0.4555 mL | 2.2774 mL | 4.5549 mL |
| 5 mM | --- | --- | --- |
| 10 mM | --- | --- | --- |
References
- [1] Thi Thanh Hanh Nguyen, et al.Flavonoid-mediated Inhibition of SARS Coronavirus 3C-like Protease Expressed in Pichia Pastoris. Biotechnol Lett. 2012 May;34(5):831-8.
- [2] Chih-Jung Kuo, et al.Engineering a Novel Endopeptidase Based on SARS 3CL(pro). Biotechniques. 2009 Dec;47(6):1029-32.
- [3] Cheng-Wei Yang, et al.Novel Small-Molecule Inhibitors of Transmissible Gastroenteritis Virus. Antimicrob Agents Chemother. 2007 Nov;51(11):3924-31.