Proteasome-activating peptide 1 TFA
PRODUCTS SPECIFICATIONS
DESCRIPTION
Overview
| MDL | - |
|---|---|
| Molecular Weight | 1632.02 |
| Molecular Formula | C65H117F3N22O15S4 |
| SMILES | - |
Summary
Proteasome-activating peptide 1 TFA is a peptide and a potent proteasome activator. Proteasome-activating peptide 1 TFA increases the chymotrypsin-like proteasomal catalytic activity and, consequently, proteolytic rates both in vitro and in culture. Proteasome-activating peptide 1 TFA prevents protein aggregation in a cellular model of amyotrophic lateral sclerosis [1] .
In Vitro
Proteasome-activating peptide 1 (TFA) (0-100 μM, 2 h) increases ChT-L catalytic activity of the 20S proteasome in a dose-dependent manner
[1]
.
Proteasome-activating peptide 1 (TFA) (50 μM, 24 h) induces the degradation of SOD1, promotes a decrease of oxidized proteins
[1]
.
Proteasome-activating peptide 1 (TFA) (0-100 μM) protects fibroblast and SH-SY5Y cells from oxidative stress
[1]
.
Proteasome-activating peptide 1 (TFA) only affects the free 20S proteasomal pool
[1]
.
Proteasome-activating peptide 1 (TFA) promotes proteasomal gate opening
[1]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay
| Cell Line: | Human neuroblastoma (SH-SY5Y) and fibroblast cells [1] |
| Concentration: | 0, 50, 100 μM (in DMEM without fetal bovine serum followed by the addition of H2O2 (500 or 750 μM) or water (0.4%)) |
| Incubation Time: | 30 min or 2 h |
| Result: | Promoted long-term proteasomal activation through interaction with the 20S core particle, increased ChT-L activity of the 20S proteasome in a dose-dependent manner, and did not reduce cell fibroblast viability. |
Western Blot Analysis
| Cell Line: | SH-SY5Y G93A cells [1] |
| Concentration: | 50 μM |
| Incubation Time: | 24 h |
| Result: | Increased the degradation of soluble SOD1, prevented the formation of SOD1-insoluble aggregates, and decreased the oxidized proteins in the total intracellular content. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
Sealed storage, away from moisture
| Powder | -80°C | 2 years |
|---|---|---|
| -20°C | 1 year |
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H 2 O : ≥ 50 mg/mL ( 30.64 mM )
* "≥" means soluble, but saturation unknown.
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 0.6127 mL | 3.0637 mL | 6.1274 mL |
| 5 mM | 0.1225 mL | 0.6127 mL | 1.2255 mL |
| 10 mM | 0.0613 mL | 0.3064 mL | 0.6127 mL |
References
- [1] Dal Vechio FH, et al. Peptides that activate the 20S proteasome by gate opening increased oxidized protein removal and reduced protein aggregation. Free Radic Biol Med. 2014;67:304-313.
- [2] Dal Vechio FH, Cerqueira F, Augusto O, Lopes R, Demasi M. Peptides that activate the 20S proteasome by gate opening increased oxidized protein removal and reduced protein aggregation. Free Radic Biol Med. 2014 Feb;67:304-13.