[CAS NO. 157654-67-6] NSC 632839

Ships within

Stock

Price

Qty

Total

$0.00

Please click "REQUEST A QUOTE" button if you need other sizes or custom synthesis

request a quote

If there is no stock, or you need other sizes or custom synthesis, please:

PRODUCTS SPECIFICATIONS [157654-67-6]

Catalog

SLK-S4922

Brand

Selleck

CAS

157654-67-6

DESCRIPTION [157654-67-6]

Overview

MDL	MFCD09753281
Molecular Weight	339.86
Molecular Formula	C21H21NO.HCl
SMILES	O=C1/C(CNC/C1=C\C2=CC=C(C)C=C2)=C/C3=CC=C(C)C=C3.[H]Cl

For research use only.

Storage

3 years,-20°C,powder
1 years,-80°C,in solvent

Shipping

Room temperature shipping(Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Concentration Volume Mass	1 mg	5 mg	10 mg
1 mM	2.9424 mL	14.7119 mL	29.4239 mL
5 mM	0.5885 mL	2.9424 mL	5.8848 mL
10 mM	0.2942 mL	1.4712 mL	2.9424 mL
50 mM	-	-	-

Description

NSC 632839 is not only a inhibitor, but also a inhibitor, it inhibits USP2, USP7, and SENP2 with of 45 μM, 37 μM, and 9.8 μM, respectively.

Features

Less potent than NSC 144303 in inducing apoptosis of E1A and E1A/C9DN cells.

Targets

SENP2 ^[1] (Cell-free assay)	USP7 ^[1] (Cell-free assay)	USP2 ^[1] (Cell-free assay)
9.8 μM(EC50)	37 μM(EC50)	45 μM(EC50)

In vitro

NSC 632839 inhibits purified USP2- and USP7-mediated cleavage of Ub-PLA2. NSC 632839 does not inhibit the reporter enzyme PLA2 over the concentration range tested (1.2–150 μM), indicating that the reported inhibition is selective for isopeptidases. NSC 632839 is capable of sustaining caspase-3/caspase-7 activity in the absence of a functional caspase-9 in E1A and E1A/C9DN cells. NSC 632839 induces apoptosis with IC50 of 15.65 μM and 16.23 μM in E1A and E1A/C9DN cells, respectively. NSC-632839 (10 μM) provokes the accumulation of polyubiquitins in E1A cells expressing HA-tagged ubiquitin. NSC-632839 (10 μM) dramatically increases the levels of Noxa and Mcl-1 in E1A cells during a time course analysis. NSC-632839 (10 μM) provokes mitochondrial fragmentation in a higher percentage of cells with an intact outer mitochondrial membrane compared with etoposide.

References

[1] Nicholson B, et al. Protein Sci, 2008, 17(6), 1035-1043.
[2] Aleo E, et al. Cancer Res, 2006, 66(18), 9235-9244.